Phosphate Buffered Saline (PBS) pH 7.4 100gm Himedia

Phosphate Buffered Saline (PBS), pH 7.4 is recommended for preparations of dilutions and blanks for the examination of samples from food, water, and other specimens. It is prepared as recommended by BAM. It is used as a diluent and sample preparation of Vibrio species Phosphates maintain the pH of the medium and sodium chloride maintains the osmotic balance.

Composition of PBS pH 7.4

• Sodium chloride 7.650 Gms / Litre
• Disodium phosphate, Anhydrous 0.724 Gms / Litre
• Dipotassium hydrogen phosphate 0.210 Gms / Litre
• Final pH ( at 25°C) 7.40 Gms / Litre
• Formula adjusted, standardized to suit performance parameters

Directions

Suspend 8.58 grams in 1000 ml purified /distilled water. Dispense in test tubes or flasks as desired. Sterilize by autoclaving at
15 lbs pressure (121°C) for 15 minutes.

Principle And Interpretation

Phosphate Buffered Saline (PBS) pH 7.4 is prepared as recommended by BAM (2). It is used as a diluent and sample preparation
of Vibrio species. Phosphates maintain the pH of the medium and sodium chloride maintains the osmotic balance.

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