Ammonium Acetate 500gm Merck, India

Ammonium acetate is a colorless, hygroscopic solid that is commonly used in various analytical and laboratory applications. It is the ammonium salt of acetic acid and is soluble in water and ethanol, making it versatile for a range of uses in chemistry.

CAS #: 631-61-8, Molar Mass: 77.08 g/mol, Grade: ACS,Reag. Ph Eur, Chemical Formula: CHCOONH, Hill Formula: CHNO, EC Number: 211-162-9

Specifications of Ammonium Acetate:

• Recomended for analysis
• Assay (acidimetric) 98.0 %
• Insoluble matter 0.005 %
• pH-value (5 %; water, 25 C) 6.7 – 7.3
• Chloride (Cl) 0.0005 %
• Nitrate (NO) 0.001 %
• Sulfate (SO) 0.001 %
• Heavy metals (as Pb) 0.0002 %
• Ca (Calcium) 0.001 %
• Fe (Iron) 0.0002 %
• Substances reducing potassium permanganate (as formic acid) 0.005 %
• Residue on ignition (as sulfate) 0.01 %
• Water 2.0 %
• Corresponds to ACS

Applications of Ammonium Acetate

1. Buffer Solutions: Ammonium acetate is frequently used to prepare buffer solutions in biochemical and molecular biology experiments due to its ability to maintain pH stability.
2. Chromatography: It is employed as an ion-pairing agent in chromatography, particularly in high-performance liquid chromatography (HPLC), to enhance the separation of analytes.
3. Protein Purification: In proteomics, ammonium acetate is used in the precipitation and purification of proteins, as it can facilitate the selective precipitation of proteins from solution.
4. Mass Spectrometry: It serves as a matrix material in mass spectrometry, helping to enhance the ionization of analytes.
5. Chemical Synthesis: Ammonium acetate is involved in various chemical reactions as a reagent.

Handling and Safety

• Personal Protective Equipment (PPE): Use gloves, safety goggles, and lab coats when handling ammonium acetate.
• Storage: Keep in a cool, dry place, in a tightly sealed container, away from incompatible substances.
• Disposal: Follow local regulations for chemical waste disposal.

Analysis

• Purity Testing: Methods like titration or chromatography can be employed to determine the purity and composition.
• pH Measurement: Prepare a solution to measure its pH, ensuring it meets the required specifications for your application.

Ammonium acetate (500 g) is a valuable chemical in analytical laboratories, particularly for its role in buffering, chromatography, and protein studies. Proper handling and adherence to safety protocols are essential for effective use.